Rapid colorimetric polymyxin B microelution directly from positive blood bottles: because patients with serious infections should not have to wait for results of culture-based methodologies

Xu L, Sun X, Ma X (2017) Systematic review and meta-analysis of mortality of patients infected with carbapenem-resistant Klebsiella pneumoniae. Ann Clin Microbiol Antimicrob 16(1):18. https://doi.org/10.1186/s12941-017-0191-3

Article  CAS  PubMed  PubMed Central  Google Scholar 

Tsuji BT, Pogue JM, Zavascki AP et al (2019) International Consensus guidelines for the optimal use of the polymyxins: endorsed by the American College of Clinical Pharmacy (ACCP), European Society of Clinical Microbiology and Infectious diseases (ESCMID), Infectious Diseases Society of America (IDSA), International Society for anti-infective pharmacology (ISAP), society of critical Care Medicine (SCCM), and Society of Infectious diseases pharmacists (SIDP). Pharmacother J Hum Pharmacol Drug Ther 39(1):10–39. https://doi.org/10.1002/phar.2209

Article  Google Scholar 

Zeng M, Xia J, Zong Z, Shi Y et al (2023) Guidelines for the diagnosis, treatment, prevention and control of infections caused by carbapenem-resistant gram-negative bacilli. J Microbiol Immunol Infect 56(4):653–671. https://doi.org/10.1016/j.jmii.2023.01.017

Article  CAS  PubMed  Google Scholar 

Paul M, Carrara E, Retamar P et al (2022) European Society of Clinical Microbiology and Infectious diseases (ESCMID) guidelines for the treatment of infections caused by multidrug-resistant Gram-negative bacilli (endorsed by European society of intensive care medicine). Clin Microbiol Infect 28(4):521–547. https://doi.org/10.1016/j.cmi.2021.11.025

Article  CAS  PubMed  Google Scholar 

Soman R, Bakthavatchalam YD, Nadarajan A et al (2021) Is it time to move away from polymyxins? Evidence and alternatives. Eur J Clin Microbiol Infect Dis 40(3):461–475. https://doi.org/10.1007/s10096-020-04053-w

Article  CAS  PubMed  Google Scholar 

Li Z, Cao Y, Yi L, Liu JH, Yang Q (2019) Emergent Polymyxin Resistance: end of an era? Open Forum Infect Dis 6(10):ofz368. https://doi.org/10.1093/ofid/ofz368

Article  CAS  PubMed  PubMed Central  Google Scholar 

Silva KED, Rossato L, Leite AF, Simionatto S (2022) Overview of polymyxin resistance in Enterobacteriaceae. Rev Soc Bras Med Trop 55:e0349–2021. https://doi.org/10.1590/0037-8682-0349-2021

Article  PubMed  PubMed Central  Google Scholar 

Kadri SS, Lai YL, Warner S et al (2021) Inappropriate empirical antibiotic therapy for bloodstream infections based on discordant in-vitro susceptibilities: a retrospective cohort analysis of prevalence, predictors, and mortality risk in US hospitals. Lancet Infect Dis 21(2):241–251. https://doi.org/10.1016/S1473-3099(20)30477-1

Article  CAS  PubMed  Google Scholar 

Nordmann P, Jayol A, Poirel L (2016) Rapid Detection of Polymyxin Resistance in Enterobacteriaceae. Emerg Infect Dis 22(6):1038–1043. https://doi.org/10.3201/eid2206.151840

Article  CAS  PubMed  PubMed Central  Google Scholar 

Barth PO, Volpato FCZ, Moreira NK, Wink PL, De Souza ÂC, Barth AL (2022) Evaluation of a rapid susceptibility test of polymyxin B by MALDI-TOF. Front Microbiol 13:1075650. https://doi.org/10.3389/fmicb.2022.1075650

Article  PubMed  PubMed Central  Google Scholar 

Collar GdaS, Raro OHF, da Silva RMC et al (2021) Polymyxin NP tests (from colonies and directly from blood cultures): accurate and rapid methodologies to detect polymyxin B susceptibility among Enterobacterales. Diagn Microbiol Infect Dis 99(4):115264. https://doi.org/10.1016/j.diagmicrobio.2020.115264

Article  CAS  PubMed  Google Scholar 

Fonseca E, Silva D, Silva-Dias A, Gomes R, Martins-Oliveira I, Ramos MH, Rodrigues AG, Cantón R, Pina-Vaz C (2019) Evaluation of rapid colistin susceptibility directly from positive blood cultures using a flow cytometry assay. Int J Antimicrob Agents 54(6):820–823. https://doi.org/10.1016/j.ijantimicag.2019.08.016

Article  CAS  Google Scholar 

Jayol A, Dubois V, Poirel L, Nordmann P (2016) Rapid Detection of Polymyxin-Resistant Enterobacteriaceae from Blood Cultures. Bourbeau P, editor. J Clin Microbiol. 54(9):2273–7. https://doi.org/10.1128/JCM.00918-16

Collar GDS, Moreira NK, Wink PL, Barth AL, Raro OHF, Dias C, De Lima Machado A, Mott MP, Caierão J (2023) Detection of polymyxins resistance among Enterobacterales: evaluation of available methods and proposal of a new rapid and feasible methodology. Ann Clin Microbiol Antimicrob 22(1):71. https://doi.org/10.1186/s12941-023-00618-7

Article  CAS  PubMed  PubMed Central  Google Scholar 

ISO (2019) ISO 20776-1:2019 susceptibility testing of infectious agents and evaluation of performance of antimicrobial susceptibility test devices – part 1: broth micro-dilution reference method for testing the in vitro activity of antimicrobial agents against rapid. Geneva, Switzerland. https://www.iso.org/standard/70464.html

Google Scholar 

Barth PO, Roesch EW, Lutz L, De Souza ÂC, Goldani LZ, Pereira DC (2023) Rapid bacterial identification by MALDI-TOF MS directly from blood cultures and rapid susceptibility testing: a simple approach to reduce the turnaround time of blood cultures. Braz J Infect Dis 27(1):102721. https://doi.org/10.1016/j.bjid.2022.102721

Article  CAS  PubMed  Google Scholar 

European Committee on Antimicrobial Susceptibility Testing (EUCAST) (2024) Methodology - EUCAST rapid antimicrobial susceptibility testing (RAST) directly from blood cultures bottles. https://www.eucast.org/. Accessed 25 March 2024

European Committee on Antimicrobial Susceptibility Testing (EUCAST) (2024) Antimicrobial susceptibility testing EUCAST disk diffusion method. https://www.eucast.org/. Accessed 18 March 2024

European Committee on Antimicrobial Susceptibility Testing (EUCAST) (2024) Breakpoint tables for interpretation of MICs and zone diameters. https://www.eucast.org/. Accessed 30 January 2024

Brazilian Committee on Antimicrobial Susceptibility Testing (BrCAST) (2023) Breakpoint tables for interpretation of MICs and zone diameters. https://www.brcast.org/. Accessed 30 January 2024

Tan X, Kim HS, Baugh K, Huang Y, Kadiyala N, Wences M, Singh N, Wenzler E, Bulman ZP (2021) Therapeutic options for Metallo-β-Lactamase-Producing Enterobacterales. Infect Drug Resist Volume 14:125–142. https://doi.org/10.2147/IDR.S246174

Article  CAS  Google Scholar 

European Committee on Antimicrobial Susceptibility Testing (EUCAST) (2016) Recommendations for MIC determination of colistin (polymyxin E) As recommended by the joint CLSI-EUCAST Polymyxin Breakpoints Working Group, https://www.eucast.org. Accessed 15 February 2024

Wink PL, Martins AS, Inamine E, Dalmolin TV, Barth AL (2019) Rapid detection of the main carbapenemases in Brazil directly from spiked blood culture using the RESIST-3 O.K.N. immunoassay. Braz J Microbiol 50(3):657–662. https://doi.org/10.1007/s42770-019-00109-y

Article  CAS  PubMed  PubMed Central  Google Scholar 

Malli E, Papagiannitsis CC, Xitsas S, Tsilipounidaki K, Petinaki E (2019) Implementation of the Rapid Polymyxin™ NP test directly to positive blood cultures bottles. Diagn Microbiol Infect Dis 95(4):114889. https://doi.org/10.1016/j.diagmicrobio.2019.114889

Article  CAS  PubMed  Google Scholar 

Luo Q, Xu L, Wang Y, Fu H, Xiao T, Yu W, Zhou W, Zhang K, Shen J, Ji J, Ying C, Xiao Y (2024) Clinical relevance, mechanisms, and evolution of polymyxin B heteroresistance carbapenem-resistant Klebsiella pneumoniae: a genomic, retrospective cohort study. Clin Microbiol Infect S1198743X:24000363. https://doi.org/10.1016/j.cmi.2024.01.014

Article  CAS  Google Scholar 

Food and Drug Administration (FDA) (2018) Antimicrobial Susceptibility Test (AST) Systems - Class II Special Controls Guidance for Industry and FDA, https://www.fda.gov. Accessed 20 February 2024

Zayet S, Lang S, Garnier P, Pierron A, Plantin J, Toko L, Royer PY, Villemain M, Klopfenstein T, Gendrin V (2021) Leclercia adecarboxylata as Emerging Pathogen in Human infections: clinical features and Antimicrobial susceptibility testing. Pathogens 10(11):1399. https://doi.org/10.3390/pathogens10111399

Article  CAS  PubMed  PubMed Central  Google Scholar 

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