Barratt JLN, Harkness J, Marriott D, Ellis JT, Stark D (2011) A review of Dientamoeba fragilis carriage in humans: several reasons why this organism should be considered in the diagnosis of gastrointestinal illness. Gut Microbes 2(1):3–12. https://doi.org/10.4161/gmic.2.1.14755

Article  PubMed  Google Scholar 

Stark D, Barratt J, Chan D, Ellis JT (2016) Dientamoeba fragilis, the neglected trichomonad of the human bowel. Clin Microbiol Rev 29(3):553–580. https://doi.org/10.1128/CMR.00076-15

Article  PubMed  PubMed Central  Google Scholar 

Garcia LS (2016) Dientamoeba fragilis, one of the neglected intestinal Protozoa. J Clin Microbiol 54(9):2243–2250. https://doi.org/10.1128/JCM.00400-16

Article  PubMed  PubMed Central  Google Scholar 

Munasinghe VS, Vella NGF, Ellis JT, Windsor PA, Stark D (2013) Cyst formation and faecal-oral transmission of Dientamoeba fragilis - the missing link in the life cycle of an emerging pathogen. Int J Parasitol 43(11):879–883. https://doi.org/10.1016/j.ijpara.2013.06.003

Article  CAS  PubMed  Google Scholar 

Stark D, Beebe N, Marriott D, Ellis J, Harkness J (2005) Prospective study of the prevalence, genotyping, and clinical relevance of Dientamoeba fragilis infections in an Australian population. J Clin Microbiol 43(6):2718–2723. https://doi.org/10.1128/JCM.43.6.2718-2723.2005

Article  CAS  PubMed  PubMed Central  Google Scholar 

Stark D, Garcia LS, Barratt JLN et al (2014) Description of Dientamoeba fragilis cyst and precystic forms from human samples. J Clin Microbiol 52(7):2680–2683. https://doi.org/10.1128/JCM.00813-14

Article  CAS  PubMed  PubMed Central  Google Scholar 

Girginkardeşler N, Kurt Ö, Kilimcioğlu AA, Ok ÜZ (2008) Transmission of Dientamoeba fragilis: evaluation of the role of Enterobius vermicularis. Parasitol Int 57(1):72–75. https://doi.org/10.1016/j.parint.2007.09.001

Article  PubMed  Google Scholar 

Girginkardeşler N, Coşkun S, Cüneyt Balcioğlu I, Ertan P, Ok UZ (2003) Dientamoeba fragilis, a neglected cause of diarrhea, successfully treated with secnidazole. Clin Microbiol Infect 9(2):110–113. https://doi.org/10.1046/j.1469-0691.2003.00504.x

Article  PubMed  Google Scholar 

Kurt O, Girginkardeşler N, Balcioğlu IC, Ozbilgin A, Ok UZ (2008) A comparison of metronidazole and single-dose ornidazole for the treatment of dientamoebiasis. Clin Microbiol Infect 14(6):601–604. https://doi.org/10.1111/j.1469-0691.2008.02002.x

Article  CAS  PubMed  Google Scholar 

Stark D, Barratt J, Ellis J, Roberts T, Marriott D, Harkness J (2010) A review of the clinical presentation of Dientamoebiasis. Am J Trop Med Hyg 82(4):614–619. https://doi.org/10.4269/ajtmh.2010.09-0478

Article  PubMed  PubMed Central  Google Scholar 

Miguel L, Salvador F, Sulleiro E et al (2018) Clinical and epidemiological characteristics of patients with dientamoeba fragilis infection. Am J Trop Med Hyg 99(5):1170–1173. https://doi.org/10.4269/AJTMH.18-0433

Article  CAS  PubMed  PubMed Central  Google Scholar 

Krogsgaard LR, Engsbro AL, Stensvold CR, Nielsen HV, Bytzer P (2015) The prevalence of intestinal parasites is not greater among individuals with irritable bowel syndrome: a population-based case-control study. Clin Gastroenterol Hepatol 13(3):507–513e2. https://doi.org/10.1016/j.cgh.2014.07.065

Article  PubMed  Google Scholar 

Brands MR, Van De Vijver E, Haisma SM, Heida A, Van Rheenen PF (2019) No association between abdominal pain and Dientamoeba in Dutch and Belgian children. Arch Dis Child 104(7):686–689. https://doi.org/10.1136/archdischild-2018-316383

Article  PubMed  Google Scholar 

Jokelainen P, Hebbelstrup Jensen B, Andreassen BU et al (2017) Dientamoeba fragilis, a Commensal in Children in Danish Day Care Centers. Gilligan P, ed. J Clin Microbiol 55(6):1707–1713. https://doi.org/10.1128/JCM.00037-17

Wong ZW, Faulder K, Robinson JL (2018) Does Dientamoeba fragilis cause diarrhea? A systematic review. Parasitol Res 117(4):971–980. https://doi.org/10.1007/s00436-018-5771-4

Article  PubMed  Google Scholar 

Belo JN, Bos ML, Brühl PC et al (2014) NHG-Standaard Acute Diarree 102–103. https://doi.org/10.1007/978-90-313-8279-8_5

Stelma F, Sauerwein RR (2020) Therapierichtlijn Parasitaire Infecties 2016

van Kalleveen MW, van Bergen M, Benninga MA, Savelkoul PHM, Plötz FB, de Meij TGJ (2021) Diagnostic and therapeutic considerations towards Dientamoeba fragilis in children: a Survey Amongst General practitioners and pediatricians in the Netherlands. J Pediatr Gastroenterol Nutr 73(6):e121–e125. https://doi.org/10.1097/MPG.0000000000003297

Article  CAS  PubMed  Google Scholar 

Malorny B, Fach P, Bunge C, Martin A, Helmuth R, Paccassoni E (2004) Diagnostic real-time PCR for detection of Salmonella in food. Appl Environ Microbiol 70(12):7046–7052. https://doi.org/10.1128/AEM.70.12.7046

Article  CAS  PubMed  PubMed Central  Google Scholar 

Tung T, Van, Canh DG, Chien BT et al (2004) Detection of Shigella by a PCR assay targeting the ipaH gene suggests increased prevalence of shigellosis in Nha Trang, Vietnam. J Clin Microbiol 42(5):2031–2035. https://doi.org/10.1128/JCM.42.5.2031

Article  PubMed Central  Google Scholar 

Lund M, Nordentoft S, Pedersen K, Madsen M (2004) Detection of Campylobacter spp. in chicken fecal samples by real-time PCR. J Clin Microbiol 42(11):5125–5132. https://doi.org/10.1128/JCM.42.11.5125-5132.2004

Article  CAS  PubMed  PubMed Central  Google Scholar 

Verweij JJ, Blangé RA, Templeton K et al (2004) Simultaneous detection of Entamoeba histolytica, Giardia lamblia, and Cryptosporidium parvum in fecal samples by using multiplex real-time PCR. J Clin Microbiol 42(3):1220–1223. https://doi.org/10.1128/JCM.42.3.1220-1223.2004

Article  CAS  PubMed  PubMed Central  Google Scholar 

Verweij JJ, Mulder B, Poell B, van Middelkoop D, Brienen EAT, van Lieshout L (2007) Real-time PCR for the detection of Dientamoeba fragilis in fecal samples. Mol Cell Probes 21(5–6):400–404. https://doi.org/10.1016/j.mcp.2007.05.006

Article  CAS  PubMed  Google Scholar 

van Gool T, Mank TG (2009) Fixatives and Permanent Stains in the Laboratory Diagnosis of Intestinal Protozoal Infections

Francis CY, Morris J, Whorwell PJ (1997) The irritable bowel severity scoring system: a simple method of monitoring irritable bowel syndrome and its progress. Aliment Pharmacol Ther 11(2):395–402. https://doi.org/10.1046/j.1365-2036.1997.142318000.x

Article  CAS  PubMed  Google Scholar 

Van Hellemond JJ, Molhoek N, Koelewijn R, Wismans PJ, van Genderen PJJ (2012) Is paromomycin the drug of choice for eradication of Dientamoeba fragilis in adults? Int J Parasitol Drugs Drug Resist 2:162–165. https://doi.org/10.1016/j.ijpddr.2012.03.002

Article  PubMed  PubMed Central  Google Scholar 

Burgaña A, Abellana R, Yordanov SZ et al (2019) Paromomycin is superior to metronidazole in Dientamoeba fragilis treatment. Int J Parasitol Drugs Drug Resist 11(November):95–100. https://doi.org/10.1016/j.ijpddr.2019.10.005

Article  PubMed  PubMed Central  Google Scholar 

Vandenberg O, Souayah H, Mouchet F, Dediste A, van Gool T (2007) Treatment of Dientamoeba fragilis with paromomycin. Pediatr Infect Dis J 26(1):88–90. https://doi.org/10.1097/01.inf.0000247139.89191.91

Article  PubMed  Google Scholar 

Clemente L, Pasut M, Carlet R, Ruscio M, Fontana F (2021) Dientamoeba fragilis in the North-East of Italy: Prevalence study and treatment. Parasitol Int 80(August 2019):102227. https://doi.org/10.1016/j.parint.2020.102227

Article  CAS  PubMed  Google Scholar 

Vandenberg O, Peek R, Souayah H et al (2006) Clinical and microbiological features of dientamoebiasis in patients suspected of suffering from a parasitic gastrointestinal illness: a comparison of Dientamoeba fragilis and Giardia lamblia infections. Int J Infect Dis 10(3):255–261. https://doi.org/10.1016/j.ijid.2005.05.011

Article  PubMed  Google Scholar 

Sperber AD, Bangdiwala SI, Drossman DA, Ghoshal UC, Simren M et al (2021) Worldwide Prevalence and Burden of Functional Gastrointestinal disorders, results of Rome Foundation Global Study. Gastroenterology 160(1):99–114e3. https://doi.org/10.1053/j.gastro.2020.04.014Epub 2020 Apr 12. PMID: 32294476