a The FKB04 chemical structure. b Liver cancer cells and liver normal cell lines were pretreated with FKB04 for 2 days, and the IC50 was calculated according to the MTT assay. c Detection of TRF2 expression in liver cancer cells and liver cells by Western blot assay. d Cell proliferation curves of Huh-7 and HepG2 in FKB04-treated cells and normal controls at indicated doses (1.0, 2.0, and 4.0 μM). e Colony formation assay in liver cancer cells treated with 1.0, 2.0, and 4.0 μM FKB04 for 10 days. f Transwell assay was used to measure the effect of FKB04 on the migration of liver cancer cells (200×). g β-Galactosidase staining method to detect senescence phenotype among cells pretreated with FKB04 (1.0, 2.0, and 4.0 μM) for 7 days. β-Galactosidase staining of 200 or more cells was quantified for each group (200×). h Quantification of g. i Western blot assays of p53, p-p53, p16, Lamin B1, and p21 protein expression levels in FKB04-treated Huh-7/HepG2 cells and normal controls at indicated doses (1.0, 2.0, and 4.0 μM) for 7 days. GAPDH was set as an internal loading control. An unpaired Student’s two-tailed t-test was performed to determine the statistical significance. ns indicates no significant -difference, *P < 0.05, **P < 0.01, ***P < 0.001.