Please use this identifier to cite or link to this item: http://nopr.niscpr.res.in/handle/123456789/65140

metadata.dc.identifier.doi: https://doi.org/10.56042/ijeb.v62i12.6850Title: Sini Decoction containing serum modulates the expression of angiogenesis-associated molecules in HUVECs by regulating the signalling pathway VEGF-Dll4/Notch1 and p38MAPK/ERK1/2/p-AKTAuthors: Qiu, Yalan
Song, Qing
Zhai, Yunyi
Wang, Lei
Cheng, Binbin
Gao, JingdongKeywords: Traditional Chinese Medicine;Medical oncology;Treatment;Vascular endothelial cellsIssue Date: Dec-2024Publisher: NIScPR-CSIR, IndiaAbstract: Angiogenesis is crucial for tumour growth and metastasis. Vascular endothelial growth factor (VEGF) and Notch ligand Delta-like 4 (Dll4) play a critical role in vessel formation and angiogenesis. This study aimed to determine anti-angiogenesis effects of Sini Decoction serum on human umbilical vein endothelial cells (HUVECs). We prepared Sini Decoction serum in doses of 0.925, 1.85, 3.7 g/mL, and administered to HUVECs. HUVECs migration was evaluated using a wound healing assay. Formation of tube-like structures in HUVECs was observed using a tube-formation assay. Gene transcriptions of Dll4, Notch1, VEGF receptor 2 (VEGFR-2) and VEGF were examined using a PCR assay. Protein expressions of Janus kinase-2/signal transducer and activator of transcription 3 (JAK2/STAT3) molecules and signal molecules downstream of VEGF/VEGFR related to angiogenesis (including mitogen-activated protein kinase (p38MAPK), extracellular signal-regulated kinase-1/2 (ERK1/2), focal adhesion kinase (FAK) and phosphorylated protein kinase B (p-Akt)) were detected by western blot. Sini Decoction serum significantly inhibited cell migration and the formation of tube-shaped structures in HUVECs compared to Control group (P0.05). In conclusion, Sini Decoction serum modulated the expression of angiogenesis-associated molecules in HUVECs by activating VEGF-Dll4/Notch1 and p38MAPK/ERK1/2/p-AKT signalling pathways.Page(s): 958-966ISSN: 0975-1009 (Online); 0019-5189 (Print)Appears in Collections:IJEB Vol.62(12) [December 2024]

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