Adler PH (2025) World blackflies (Diptera: Simuliidae): a comprehensive revision of the taxonomic and geographical inventory [2025]. Department of Plant and Environmental Sciences, Clemson University, Clemson, South Carolina, USA. https://biomia.sites.clemson.edu/pdfs/blackflyinventory.pdf

An Y, Li C, Li J, Wang Y (2022) The complete mitochondrial genome of Simulium jisigouense (Diptera: Simuliidae) and phylogenetic analysis of Simuliidae. Front Ecol Evol 10:152–160. https://doi.org/10.3389/fevo.2022.932601

Article  Google Scholar 

Andrade-Souza V, Silva JG, Hamada N (2017) Phylogeography and population diversity of Simulium hirtipupa Lutz (Diptera: Simuliidae) based on mitochondrial COI sequences. PLoS ONE 12:e0190091. https://doi.org/10.1371/journal.pone.0190091

Article  CAS  PubMed  PubMed Central  Google Scholar 

Bankevich A, Nurk S, Antipov D, Gurevich AA, Dvorkin M, Kulikov AS, Lesin VM, Nikolenko SI, Pham S, Prjibelski AD, Pyshkin AV, Sirotkin AV, Vyahhi N, Tesler G, Alekseyev MA, Pevzner PA (2012) SPAdes: a new genome assembly algorithm and its applications to single-cell sequencing. J Comput Biol 19:455–477. https://doi.org/10.1089/cmb.2012.0021

Article  CAS  PubMed  PubMed Central  Google Scholar 

Beckenbach AT (2012) Mitochondrial genome sequences of Nematocera (lower Diptera): evidence of rearrangement following a complete genome duplication in a winter crane fly. Genome Biol Evol 4:89–101. https://doi.org/10.1093/gbe/evr131

Article  CAS  PubMed  Google Scholar 

Beckenbach AT, Borkent A (2003) Molecular analysis of the biting midges (Diptera: Ceratopogonidae), based on mitochondrial cytochrome oxidase subunit 2. Mol Phylogenet Evol 27:21–35. https://doi.org/10.1016/s1055-7903(02)00395-0

Article  CAS  PubMed  Google Scholar 

Bertone MA, Courtney GW, Wiegmann BM (2008) Phylogenetics and Temporal diversification of the earliest true flies (Insecta: Diptera) based on multiple nuclear genes. Syst Entomol 33:668–687. https://doi.org/10.1111/j.1365-3113.2008.00437.x

Article  Google Scholar 

Birney E, Durbin R (2000) Using genewise in the Drosophila annotation experiment. Genome Res 10:547–548. https://doi.org/10.1101/gr.10.4.547

Article  CAS  PubMed  PubMed Central  Google Scholar 

Blagoderov V, Grimaldi DA, Fraser NC (2007) How time flies for flies, diverse Diptera from the triassic of Virginia and early radiation of the order. Am Mus Novit 5:1–39

Article  Google Scholar 

Bolger AM, Lohse M, Usadel B (2014) Trimmomatic: a flexible trimmer for illumina sequence data. Bioinformatics 30:2114–2120. https://doi.org/10.1093/bioinformatics/btu170

Article  CAS  PubMed  PubMed Central  Google Scholar 

Borkent A (2012) The pupae of Culicomorpha–morphology and a new phylogenetic tree. Zootaxa 3396:1–98. https://doi.org/10.11646/zootaxa.3396.1.1

Article  Google Scholar 

Briscoe AG, Sivell D, Harbach RE (2017) The complete mitochondrial genome of Dixella aestivalis (Diptera: nematocera: Dixidae). Mitochondrial DNA DNA Mapp Seq Anal 28:83–84. https://doi.org/10.3109/19401736.2015.1110809

Article  CAS  Google Scholar 

Camacho C, Coulouris G, Avagyan V, Ma N, Papadopoulos J, Bealer K, Madden TL (2009) BLAST+: architecture and applications. BMC Bioinformatics 10:421. https://doi.org/10.1186/1471-2105-10-421

Article  CAS  PubMed  PubMed Central  Google Scholar 

Cameron SL (2014) Insect mitochondrial genomics: implications for evolution and phylogeny. Annu Rev Entomol 59:95–117. https://doi.org/10.1146/annurev-ento-011613-162007

Article  CAS  PubMed  Google Scholar 

Cameron SL (2025) Insect mitochondrial genomics: A decade of progress. Annu Rev Entomol 70:83–101. https://doi.org/10.1146/annurev-ento-013024-015553

Article  CAS  PubMed  Google Scholar 

Cameron SL, Lambkin CL, Barker SC, Whiting MF (2007) A mitochondrial genome phylogeny of diptera: whole genome sequence data accurately resolve relationships over broad timescales with high precision. Syst Entomol 32:40–59

Article  Google Scholar 

Chen H (2016) Chinese blackflies (Diptera: Simuliidae). Science, Beijing

Google Scholar 

Chen Q, Chen L, Liao CQ, Wang X, Wang M, Huang GH (2022) Comparative mitochondrial genome analysis and phylogenetic relationship among lepidopteran species. Gene 830:146516. https://doi.org/10.1016/j.gene.2022.146516

Article  CAS  PubMed  Google Scholar 

Chiriac A, Brzezinski P, Miron L, Moldovan C, Podoleanu C, Stolnicu S (2016) Simuliosis–A dermatosis caused by black flies. Allergol Int 65:217–218. https://doi.org/10.1016/j.alit.2015.11.005

Article  CAS  PubMed  Google Scholar 

Conflitti IM, Pruess KP, Cywinska A, Powers TO, Currie DC (2013) DNA barcoding distinguishes pest species of the black fly genus Cnephia (Diptera: Simuliidae). J Med Entomol 50:1250–1260. https://doi.org/10.1603/me13063

Article  CAS  PubMed  Google Scholar 

Crosskey RW, Howard TM (1987) A new taxonomic and geographical inventory of world blackflies (Diptera: Simuliidae). Department of Entomology, the Natural History Museum, London, p 145

Google Scholar 

Cui X, Lu Z, Wang S, Jing-Yan Wang J, Gao X (2016) CMsearch: simultaneous exploration of protein sequence space and structure space improves not only protein homology detection but also protein structure prediction. Bioinformatics 32:i332–i340. https://doi.org/10.1093/bioinformatics/btw271

Article  CAS  PubMed  PubMed Central  Google Scholar 

Fang Y, Sun M, Fang Y, Zuo Z, Liu L, Chu L, Ding L, Hu C, Li F, Han R, Xia X, Zhou S, Sun E (2022) Complete mitochondrial genomes of Thyreophagus entomophagus and Acarus Siro (Sarcoptiformes: Astigmatina) provide insight into mitogenome features, evolution, and phylogeny among Acaroidea mites. Exp Appl Acarol 88:57–74. https://doi.org/10.1007/s10493-022-00745-4

Article  CAS  PubMed  Google Scholar 

Farkas J (1984) Simuliosis. Analysis of dermatological manifestations following blackfly (Simuliidae) bites as observed in the years 1981–1983 in Bratislava (Czechoslovakia). Derm Beruf Umwelt 32:171–173

CAS  PubMed  Google Scholar 

Friedrich M, Tautz D (1997) Evolution and phylogeny of the diptera: a molecular phylogenetic analysis using 28S rDNA sequences. Syst Biol 46:674–698. https://doi.org/10.1093/sysbio/46.4.674

Article  CAS  PubMed  Google Scholar 

Guo ZQ, Gao YJ, Chen YX, Zhan LM, Storey KB, Yu DN, Zhang JY (2024) Comparative mitogenome of phylogenetic relationships and divergence time analysis within Potamanthidae (Insecta: Ephemeroptera). Insects 15:357. https://doi.org/10.3390/insects15050357

Article  PubMed  PubMed Central  Google Scholar 

Gyasi ME, Okonkwo ON, Tripathy K (2024) Onchocerciasis. Treasure Island (FL): StatPearls Publishing. https://www.ncbi.nlm.nih.gov/books/NBK559027/

Hennig W (1973) Diptera (Zweiflügler). Handbuch Der Zool 4:1–337

Google Scholar 

Jühling F, Pütz J, Bernt M, Donath A, Middendorf M, Florentz C, Stadler PF (2012) Improved systematic tRNA gene annotation allows new insights into the evolution of mitochondrial tRNA structures and into the mechanisms of mitochondrial genome rearrangements. Nucleic Acids Res 40:2833–2845. https://doi.org/10.1093/nar/gkr1131

Article  CAS  PubMed  Google Scholar 

Kang Z, Li X, Yang D (2016) The complete mitochondrial genome of Dixella Sp. (Diptera: nematocera, Dixidae). Mitochondrial DNA A DNA Mapp Seq Anal 27:1528–1529. https://doi.org/10.3109/19401736.2014.953123

Article  CAS  Google Scholar 

Kang Z, Zhang X, Ding S, Tang C, Wang Y, Jong H, Cameron SL, Wang M, Yang D (2017) Transcriptomes of three species of Tipuloidea (Diptera, Tipulomorpha) and implications for phylogeny of Tipulomorpha. PLoS ONE 12:e0173207. https://doi.org/10.1371/journal.pone.0173207

Article  CAS  PubMed  PubMed Central  Google Scholar 

Kocher A, Gantier JC, Holota H, Jeziorski C, Coissac E, Bañuls AL, Girod R, Gaborit P, Murienne J (2016) Complete mitochondrial genome of Lutzomyia (Nyssomyia) umbratilis (Diptera: Psychodidae), the main vector of Leishmania guyanensis. Mitochondrial DNA A DNA Mapp Seq Anal 27:4219–4221. https://doi.org/10.3109/19401736.2015.1022748

Article  CAS