A method for determination of ten kinds of sweeteners in soybean products by multi-plug filtration cleanup (m-PFC) combined with ultra-high-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) was established. The samples were ultrasonically extracted after vortex by 1% formic acid-acetonitrile, degreased by n-hexane liquid-liquid extraction using and purified by solid phase extraction using m-PFC column (Oasis PRiME HLB). The analytes were separated by a Waters ACQUITY UPLC® BEH C18 (2.1 mm × 150 mm, 1.7 μm) column, and gradient eluted with a mixed solution of (5 mmol/L ammonium formate+0.1% formic acid) and acetonitrile. The mass spectrometric acquisition was performed by multiple-reaction monitoring (MRM) in both negative and positive ionization mode. Quantitative analysis was performed using an external standard method with matrix-matched calibration curves. Under optimal conditions, a total of ten target compounds were well separated within 10 min, and a good linearity was obtained in the concentration ranges of 0.5-20 μg L-1 with correlation coefficients of greater than 0.999. The limits of detection (LODs) and quantification (LOQs) were 0.5 μg kg-1 and 1.0 μg kg-1, respectively. The recoveries of the ten sweeteners in seven kinds of soybean products ranged from 80.6% to 119.4% at low, medium and high spiked levels (1.0 μg kg-1, 5.0 μg kg-1 and 10 μg kg-1), with precisions (RSD) in the range of 0.05%-14.5%. This method is simple, rapid, accurate and sensitive, which greatly improves the efficiency of sample analysis and the accuracy of qualitative and quantitative analysis results of the target substances. It could meet the needs of quantitative analysis and qualitative screening of sweeteners in bulk soybean products samples, and provide technical support for rapid analysis of sweeteners in soybean products. It is of great significance for the analysis of trace components of sweeteners in soybean products.

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