Materials

Preparation of curcumin-loaded transethosome formulation

Measurement of vesicular size, Polydispersity Index (PDI), and zeta potential

pH measurements

Encapsulation efficiency determination

Transmission Electron Microscopy (TEM) analysis

Differential Scanning Calorimetry (DSC) analysis

Stability evaluation

Human skin permeation and deposition experiments

The preparation of the Skin

Ex-vivo skin permeation studies

Ex-vivo skin deposition studies

HPLC analysis conditions

ATR-FTIR spectroscopy analysis

Antimicrobial activity assessment

In vitro Cytotoxicity assay

In vitro wound healing assay/ In vitro scratch wound closure assay

Statistical analyses