Comparison of HDL Cholesterol Efflux Capacity Assays and the Effect of Assay Selection on CEC and Coronary Heart Disease

Thakkar H, Vincent V, Shukla S, Sra M, Kanga U, Aggarwal S, et al. Improvements in cholesterol efflux capacity of HDL and adiponectin contribute to mitigation in cardiovascular disease risk after bariatric surgery in a cohort with morbid obesity. Diabetol Metab Syndr. 2021;13(1):46.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Rohatgi A, Khera A, Berry JD, Givens EG, Ayers CR, Wedin KE, et al. HDL cholesterol efflux capacity and incident cardiovascular events. N Engl J Med. 2014;371(25):2383–93.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Horiuchi Y, Lai SJ, Kameda T, Tozuka M, Ohkawa R. Comparison of a novel cholesterol efflux assay using immobilized liposome-bound gel beads with the conventional method. Biosci Rep. 2020;40(8):BSR20201495.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Pérez-Méndez Ó, Pacheco HG, Martínez-Sánchez C, Franco M. HDL-cholesterol in coronary artery disease risk: function or structure? Clin Chim Acta Int J Clin Chem. 2014;15(429):111–22.

Article  Google Scholar 

Cardner M, Yalcinkaya M, Goetze S, Luca E, Balaz M, Hunjadi M, et al. Structure-function relationships of HDL in diabetes and coronary heart disease. JCI Insight. 2020;5(1):e131491.

Article  PubMed  PubMed Central  Google Scholar 

Horiuchi Y, Lai SJ, Yamazaki A, Nakamura A, Ohkawa R, Yano K, et al. Validation and application of a novel cholesterol efflux assay using immobilized liposomes as a substitute for cultured cells. Biosci Rep. 2018;38(2):BSR20180144.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Lewis GF, Rader DJ. New insights into the regulation of HDL metabolism and reverse cholesterol transport. Circ Res. 2005;96(12):1221–32.

Article  CAS  PubMed  Google Scholar 

Groenen AG, Halmos B, Tall AR, Westerterp M. Cholesterol efflux pathways, inflammation, and atherosclerosis. Crit Rev Biochem Mol Biol. 2021;56(4):426–39.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Horiuchi Y, Lai SJ, Kameda T, Tozuka M, Ohkawa R. Novel cholesterol efflux assay using immobilized liposome-bound gel beads: confirmation and improvement for application in clinical laboratory. Ann Clin Biochem. 2022;59(2):134–43.

Article  CAS  PubMed  Google Scholar 

Holzer M, Ljubojevic-Holzer S, Souza Junior DR, Stadler JT, Rani A, Scharnagl H, et al. HDL isolated by immunoaffinity, ultracentrifugation, or precipitation is compositionally and functionally distinct. J Lipid Res. 2022;63(12): 100307.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Schachtl-Riess JF, Coassin S, Lamina C, Demetz E, Streiter G, Hilbe R, et al. Lysis reagents, cell numbers, and calculation method influence high-throughput measurement of HDL-mediated cholesterol efflux capacity. J Lipid Res. 2021;62: 100125.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Sankaranarayanan S, Kellner-Weibel G, de la Llera-Moya M, Phillips MC, Asztalos BF, Bittman R, et al. A sensitive assay for ABCA1-mediated cholesterol efflux using BODIPY-cholesterol. J Lipid Res. 2011;52(12):2332–40.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Rhainds D, Tardif JC. From HDL-cholesterol to HDL-function: cholesterol efflux capacity determinants. Curr Opin Lipidol. 2019;30(2):101–7.

Article  CAS  PubMed  Google Scholar 

Kiss RS, Maric J, Marcel YL. Lipid efflux in human and mouse macrophagic cells: evidence for differential regulation of phospholipid and cholesterol efflux. J Lipid Res. 2005;46(9):1877–87.

Article  CAS  PubMed  Google Scholar 

Thakkar H, Vincent V, Roy A, Singh S, Ramakrishnan L, Kalaivani M, et al. HDL functions and their interaction in patients with ST elevation myocardial infarction: a case control study. Lipids Health Dis. 2020;19(1):75.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Ebtehaj S, Gruppen EG, Bakker SJL, Dullaart RPF, Tietge UJF. HDL (High-density lipoprotein) cholesterol efflux capacity is associated with incident cardiovascular disease in the general population. Arterioscler Thromb Vasc Biol. 2019;39(9):1874–83.

Article  CAS  PubMed  Google Scholar 

Kosmas CE, Martinez I, Sourlas A, Bouza KV, Campos FN, Torres V, et al. High-density lipoprotein (HDL) functionality and its relevance to atherosclerotic cardiovascular disease. Drugs Context. 2018;7: 212525.

Article  PubMed  PubMed Central  Google Scholar 

Shah AS, Tan L, Long JL, Davidson WS. Proteomic diversity of high density lipoproteins: our emerging understanding of its importance in lipid transport and beyond. J Lipid Res. 2013;54(10):2575–85.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Kontush A, Lhomme M, Chapman MJ. Unraveling the complexities of the HDL lipidome. J Lipid Res. 2013;54(11):2950–63.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Fernández-Castillejo S, Rubió L, Hernáez Á, Catalán Ú, Pedret A, Valls RM, et al. Determinants of HDL cholesterol efflux capacity after virgin olive oil ingestion: interrelationships with fluidity of HDL monolayer. Mol Nutr Food Res. 2017;61(12):1700445.

Article  Google Scholar 

Papagiannis A, Gkolfinopoulou C, Tziomalos K, Dedemadi AG, Polychronopoulos G, Milonas D, et al. HDL cholesterol efflux capacity and phospholipid content are associated with the severity of acute ischemic stroke and predict its outcome. Clin Chim Acta Int J Clin Chem. 2023;1(540): 117229.

Article  Google Scholar 

Horiuchi Y, Ohkawa R, Lai SJ, Shimano S, Hagihara M, Tohda S, et al. Usefulness of apolipoprotein B-depleted serum in cholesterol efflux capacity assays using immobilized liposome-bound gel beads. Biosci Rep. 2019;39(4):BSR20190213.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Munroe WH, Phillips ML, Schumaker VN. Excessive centrifugal fields damage high density lipoprotein [S]. J Lipid Res. 2015;56(6):1172–81.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Hafiane A, Genest J. HDL-mediated cellular cholesterol efflux assay method. Ann Clin Lab Sci. 2015;45(6):659–68.

CAS  PubMed  Google Scholar 

Comments (0)

No login
gif