Novel method of paraffin embedding cultured cells and organoids using silicone molds

Aisagbonhi O, Birungi A, Atwine R, Behayo P, Ayebaziwe B, Roberts D, Tambouret R (2018) Modified plasma-thrombin method of cell block preparation for fine-needle aspiration biopsies in resource-limited settings. Am J Clin Patho 150(2):137–145

Article  CAS  Google Scholar 

Edfors F, Hober A, Linderbäck K, Maddalo G, Azimi A, Sivertsson Å, Tegel H, Hober S, Szigyarto CA-K, Fagerberg L, von Feilitzen K, Oksvold P, Lindskog C, Forsström B, Uhlen M (2018) Enhanced validation of antibodies for research applications. Nat Commun 9(1):4130

Article  PubMed  PubMed Central  Google Scholar 

Fritz AJ, Hong D, Boyd J, Kost J, Finstaad KH, Fitzgerald MP, Hanna S, Abuarqoub AH, Malik M, Bushweller J, Tye C, Ghule P, Gordon J, Frietze S, Zaidi SK, Lian JB, Stein JL, Stein GS (2020) RUNX1 and RUNX2 transcription factors function in opposing roles to regulate breast cancer stem cells. J Cell Physiol 235(10):7261–7272

Article  CAS  PubMed  PubMed Central  Google Scholar 

Gambardella G, Viscido G, Tumaini B et al (2022) A single-cell analysis of breast cancer cell lines to study tumour heterogeneity and drug response. Nat Commun 13:1714. https://doi.org/10.1038/s41467-022-29358-6

Article  CAS  PubMed  PubMed Central  Google Scholar 

Havnar C, Hotzel K, Espiritu C, Lo A, Webster JD (2022) Standardized processing for formalin-fixed, paraffin-embedded cell pellet immunohistochemistry controls. JoVE 185:e64276

Google Scholar 

Hong D, Messier TL, Tye CE, Dobson JR, Fritz AJ, Sikora KR, Browne G, Stein JL, Lian JB, Stein GS (2017) Runx1 stabilizes the mammary epithelial cell phenotype and prevents epithelial to mesenchymal transition. Oncotarget 8(11):17610–17627

Article  PubMed  PubMed Central  Google Scholar 

Mazzucchelli S, Piccotti F, Allevi R et al (2019) Establishment and morphological characterization of patient-derived organoids from breast cancer. Biol Proced Online 21:12. https://doi.org/10.1186/s12575-019-0099-8

Article  CAS  PubMed  PubMed Central  Google Scholar 

Moskaluk CA, Stoler MH (2002) Agarose mold embedding of cultured cells for tissue microarrays. Diagn Mol Pathol 11(4):234–238

Article  PubMed  Google Scholar 

Rekhtman N, Buonocore DJ, Rudomina D, Friedlander M, Dsouza C, Aggarwal G, Arcila M, Edelweiss M, Lin O (2017) Novel modification of histogel-based cell block preparation method: improved sufficiency for molecular studies. Arch Pathol Lab Med 142(4):529–535

Article  PubMed  PubMed Central  Google Scholar 

Srebotnik Kirbis I, Kholova I, Huhtala H, Bongiovanni M, Strojan Flezar M, Hodgson C, Cochand-Priollet B (2024) Cell block practices in European cytopathology laboratories. Cancer Cytopathol 132(4):250–259

Article  CAS  PubMed  Google Scholar 

Uhlen M, Bandrowski A, Carr S, Edwards A, Ellenberg J, Lundberg E, Rimm DL, Rodriguez H, Hiltke T, Snyder M, Yamamoto T (2016) A proposal for validation of antibodies. Nat Methods 13(10):823–827

Article  CAS  PubMed  PubMed Central  Google Scholar 

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