Jimeno B, Rubalcaba JG. Modelling the role of glucocorticoid receptor as mediator of endocrine responses to environmental challenge. Philos Trans R Soc Lond B Biol Sci. 2024;379(1898):20220501. https://doi.org/10.1098/rstb.2022.0501.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Luo J, Zhou C, Wang S, Tao S, Liao Y, Shi Z, Tang Z, Wu Y, Liu Y, Yang P. Cortisol synergizing with endoplasmic reticulum stress induces regulatory T-cell dysfunction. Immunology. 2023;170(3):334–43. https://doi.org/10.1111/imm.13669.

Article  CAS  PubMed  Google Scholar 

El-Farhan N, Rees DA, Evans C. Measuring cortisol in serum, urine and saliva - are our assays good enough? Ann Clin Biochem. 2017;54(3):308–22. https://doi.org/10.1177/0004563216687335.

Article  CAS  PubMed  Google Scholar 

Choi MH. Clinical and technical aspects in free cortisol measurement. Endocrinol Metab (Seoul). 2022;37(4):599–607. https://doi.org/10.3803/EnM.2022.1549.

Article  CAS  PubMed  Google Scholar 

Mu D, Fang J, Yu S, Ma Y, Cheng J, Hu Y, Song A, Zhao F, Zhang Q, Qi Z, Zhang K, Xia L, Qiu L, Zhu H, Cheng X. Comparison of direct and extraction immunoassay methods with liquid chromatography-tandem mass spectrometry measurement of urinary free cortisol for the diagnosis of Cushing’s syndrome. Ann Lab Med. 2024;44(1):29–37. https://doi.org/10.3343/alm.2024.44.1.29.

Article  CAS  PubMed  Google Scholar 

Oster H, Damerow S, Kiessling S, Jakubcakova V, Abraham D, Tian J, Hoffmann MW, Eichele G. The circadian rhythm of glucocorticoids is regulated by a gating mechanism residing in the adrenal cortical clock. Cell Metab. 2006;4(2):163–73. https://doi.org/10.1016/j.cmet.2006.07.002.

Article  CAS  PubMed  Google Scholar 

Lightman SL, Birnie MT, Conway-Campbell BL. Dynamics of ACTH and cortisol secretion and implications for disease. Endocr Rev. 2020;41(3):bnaa002. https://doi.org/10.1210/endrev/bnaa002.

Article  PubMed  PubMed Central  Google Scholar 

Oßwald A, Wang R, Beuschlein F, Hartmann MF, Wudy SA, Bidlingmaier M, Zopp S, Reincke M, Ritzel K. Performance of LC-MS/MS and immunoassay based 24-h urine free cortisol in the diagnosis of Cushing’s syndrome. J Steroid Biochem Mol Biol. 2019;190:193–7. https://doi.org/10.1016/j.jsbmb.2019.04.004.

Article  CAS  PubMed  Google Scholar 

Perrin P, Plotton I, Berthiller J, Borson-Chazot F, Raverot G, Raverot V. Urinary free cortisol: an automated immunoassay without extraction for diagnosis of Cushing’s syndrome and follow-up of patients treated by anticortisolic drugs. Clin Endocrinol (Oxf). 2020;93(1):76–8. https://doi.org/10.1111/cen.14200.

Article  PubMed  Google Scholar 

Guo W, Li Y, Jiang N, Wu JXW. Is clinical mass spectrometry the future of hormone testing?(Chinese). Chin J Lab Med. 2024;47(07):717–21.

Google Scholar 

Huang H, Xie F, Zhang F, Xiong YMT. Determination of urinary free cortisol by RIA and establishment of normal reference range. Sichuan Med. 2005;26(3):285–6.

CAS  Google Scholar 

Zhu Q, Zhu F, Zhao J, Xu G, Shao J, Cao RHL. Establishment and performance evaluation of liquid-chromatography tandem-mass spectrometry for determining serum cortisone. Lab Med. 2017;32(6):524–30.

Google Scholar 

Ray JA, Kish-Trier E, Johnson LM. Measurement of urinary free cortisol and cortisone by LC-MS/MS. Methods Mol Biol. 2022;2546:119–28. https://doi.org/10.1007/978-1-0716-2565-1_11.

Article  CAS  PubMed  Google Scholar 

Grau J, Benede JL, Chisvert A, Salvador A. Modified magnetic-based solvent-assisted dispersive solid-phase extraction: application to the determination of cortisol and cortisone in human saliva. J Chromatogr A. 2021;1652:462361. https://doi.org/10.1016/j.chroma.2021.462361.

Article  CAS  PubMed  Google Scholar 

Bonnet-Serrano F, Nakib S, Zientek C, Guignat L, Guibourdenche J, Bertherat J, Menet MC. Urinary free cortisol determination and interferences studies using liquid chromatography coupled to tandem mass spectrometry after on-line solid phase extraction based on Turboflow(TM) chromatography. Metabolites. 2023;13(10). https://doi.org/10.3390/metabo13101063.

Bianchi L, Campi B, Sessa MR, De Marco G, Ferrarini E, Zucchi R, Marcocci C, Vitti P, Manetti L, Saba A, Agretti P. Measurement of urinary free cortisol by LC-MS-MS: adoption of a literature reference range and comparison with our current immunometric method. J Endocrinol Invest. 2019;42(11):1299–305. https://doi.org/10.1007/s40618-019-01050-5.

Article  CAS  PubMed  Google Scholar 

Turpeinen U, Hämäläinen E. Determination of cortisol in serum, saliva and urine. Best Pract Res Clin Endocrinol Metab. 2013;27(6):795–801. https://doi.org/10.1016/j.beem.2013.10.008.

Article  CAS  PubMed  Google Scholar 

Wu Y, Huang W, Jia C, Hu DWL. Improvement of extraction method and analysis of the interferences in the detection of urinary free cortisol. J Tianjin Med Univ. 2022;28(4):436–41.

Google Scholar 

Wu M, Zhao D, Gu B, Wang Z, Hu J, Yu Z, Yu J. Efficient degradation of aqueous dichloromethane by an enhanced microbial electrolysis cell: degradation kinetics, microbial community and metabolic mechanisms. J Environ Sci (China). 2024;139:150–9. https://doi.org/10.1016/j.jes.2023.05.029.

Article  CAS  PubMed  Google Scholar 

Vidal S. Safety first: a recent case of a dichloromethane injection injury. ACS Cent Sci. 2020;6(2):83–6. https://doi.org/10.1021/acscentsci.0c00100.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Li J, Zheng X, Zhao R, Liu MJZ. Validation of the performance of ethyl acetate extraction for free cortisol in urine. Med Laboratory Sci and Clin. 2021;32(3):35–40.

Google Scholar 

Zhu G, Xiao Z, Zhu G. Fabrication and characterization of ethyl acetate-hydroxypropyl-beta-cyclodextrin inclusion complex. J Food Sci. 2021;86(8):3589–97. https://doi.org/10.1111/1750-3841.15835.

Article  CAS  PubMed  Google Scholar 

Shang Z, Cai W, Cao Y, Wang F, Wang Z, Lu J, Zhang J. An integrated strategy for rapid discovery and identification of the sequential piperine metabolites in rats using ultra high-performance liquid chromatography/high resolution mass spectrometery. J Pharm Biomed Anal. 2017;146:387–401. https://doi.org/10.1016/j.jpba.2017.09.012.

Article  CAS  PubMed  Google Scholar 

Rezaei F, Eikani MH, Nosratinia F, Bidaroni HH. Optimization of ethanol-modified subcritical water extraction of curcuminoids from turmeric (Curcuma longa L.) rhizomes: comparison with conventional techniques. Food Chem. 2023;410:135331. https://doi.org/10.1016/j.foodchem.2022.135331.

Article  CAS  PubMed  Google Scholar 

Allay A, Benkirane C, Ben Moumen A, Fauconnier ML, Bouakline H, Nkengurutse J, Serghini Caid H, Elamrani A, Mansouri F. Optimizing ethanol-modified supercritical CO(2) extraction for enhanced bioactive compound recovery in hemp seed oil. Sci Rep. 2025;15(1):8551. https://doi.org/10.1038/s41598-025-91441-x.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Niu Y, Yang Y, Mao C, Xiao Z. Effects of gallic acid on the release of aroma compounds in Moutai Baijiu. Food Res Int. 2024;176:113655. https://doi.org/10.1016/j.foodres.2023.113655.

Article  CAS  PubMed  Google Scholar 

Lin Y, Huang J, Hu Y, Huang Y, Gao Q. Methodology verification of electrochemical luminescence immunoassay for determination 0f 24 hours urinary free cortisol (Chinese). Labeled Immunoassays & Clin Med. 2014;21(3):306–9.

CAS  Google Scholar 

Casals G, Ballesteros MA, Zamora A, Martinez I, Fernandez-Varo G, Mora M, Hanzu FA, Morales-Ruiz M (2024) LC-HRMS and GC-MS profiling of urine free cortisol, cortisone, 6beta-, and 18-hydroxycortisol for the evaluation of glucocorticoid and mineralocorticoid disorders. Biomolecules 14(5). https://doi.org/10.3390/biom14050558

Luo W, Dong Z, Jia C, Huang X, Hao Q, Xie H, Chai M, Wang X, Cao F, Dong L. A urinary cortisol extraction composition, extraction method and extraction device (Chinese). 2024. CN202410430467[P][2024–07–25].

Horowitz GL. Reference intervals: Practical Aspects. EJIFCC. 2008;19(2):95–105.

PubMed  PubMed Central  Google Scholar 

Gu M, Sun Y, Jia C, Fan M, Rrn N, Zhang H, Yuan HWL. Comparability of routine biochemical indexes between dry chemistry and wet chemistry analysis systems (Chinese). Laboratory Medicine. 2019;34(12):1128–32.

Google Scholar