Keeping track of caffeine everyday consumption: high-performance thin-layer chromatography‒densitometric determination of caffeine in beverages and chocolates

Susan B, O’Neil Maryadelle J, Ann S, Heckelman Patricia E, Obenchain John R, Gallipeau Jo Ann R, Ann DM (2001) The Merck Index: an encyclopaedia of chemicals, drugs, and biologicals. Merck & Co. Inc, Whitehouse Station

Google Scholar 

Wanyika HN, Gatebe EG, Gitu LM, Ngumba EK, Maritim CW (2010) Determination of caffeine content of tea and instant coffee brands found in the Kenyan market. Afr J Food Sci 4:353–358

CAS  Google Scholar 

Barcelos RP, Lima FD, Carvalho NR, Bresciani G, Royes LF (2020) Caffeine effects on systemic metabolism, oxidative-inflammatory pathways, and exercise performance. Nutr Res 80:1–17. https://doi.org/10.1016/j.nutres.2020.05.005

Article  PubMed  CAS  Google Scholar 

Maughan RJ (2018) IOC medical and scientific commission reviews its position on the use of dietary supplements by elite athletes. Br J Sports Med 52:418–419. https://doi.org/10.1136/bjsports-2018-099199

Article  PubMed  Google Scholar 

Miller KE, Quigley BM (2011) Energy drink use and substance use among musicians. J Caffeine Res 1:67–73. https://doi.org/10.1089/jcr.2011.0003

Article  Google Scholar 

Norton TR, Lazev AB, Sullivan MJ (2011) The “buzz” on caffeine: patterns of caffeine use in a convenience sample of college students. J Caffeine Res 1:35–40. https://doi.org/10.1089/jcr.2010.0003

Article  Google Scholar 

Lane JD (2011) Caffeine, glucose metabolism, and type 2 diabetes. J Caffeine Res 1:23–28. https://doi.org/10.1089/jcr.2010.0007

Article  CAS  Google Scholar 

Mohamed Mekky H (2015) Evaluation of tea and coffee products commercialized in Egypt using HPTLC. Zagazig J Pharm Sci 24:88–96. https://doi.org/10.21608/zjps.2015.38165

Article  Google Scholar 

Tzanavaras PD, Themelis DG (2007) Development and validation of a high-throughput high-performance liquid chromatographic assay for the determination of caffeine in food samples using a monolithic column. Anal Chim Acta 581:89–94. https://doi.org/10.1016/j.aca.2006.07.081

Article  PubMed  CAS  Google Scholar 

Regulation (EU) No 1169/2011 of the European Parliament and of the Council of 25 October 2011 on the provision of food information to consumers. In: EUR-Lex. http://data.europa.eu/eli/reg/2011/1169/2025-04-01. Accessed 7 July 2025

Bensa M, Vovk I, Glavnik V (2023) Resveratrol food supplement products and the challenges of accurate label information to ensure food safety for consumers. Nutrients 15:474. https://doi.org/10.3390/nu15020474

Article  PubMed  PubMed Central  CAS  Google Scholar 

O’Brien MC, Arria AM, Howland J, James JE, Marczinski CA (2011) Caffeine, alcohol, and youth: a toxic mix. J Caffeine Res 1:15–21. https://doi.org/10.1089/jcr.2011.1202

Article  PubMed  PubMed Central  Google Scholar 

National Institute of Public Health (2023) Energijske pijače niso za otroke in mladostnike. In: NIJZ. https://nijz.si/mediji/energijske-pijace-niso-za-otroke-in-mladostnike/. Accessed 14 July 2025

Jain D, Misra H, Mehta D, Mehta B, Soni M (2009) Study of extraction and HPTLC - UV method for estimation of caffeine in marketed tea (Camellia sinensis) granules. Int J Green Pharm 3:47. https://doi.org/10.4103/0973-8258.49374

Article  Google Scholar 

Vovk I, Golc-Wondra A, Prošek M (1997) Validation of an HPTLC method for determination of caffeine. JPC – J Planar Chromatogr – Mod TLC 10:416–419

Prošek M, Golc-Wondra A, Vovk I, Andrenšek S (2000) Quantification of caffeine by off-line TLC-MS. JPC – J Planar Chromatogr – Mod TLC 13:452–456

Palacios C, Salatino ML, Salatino A (2017) TLC procedure for determination of approximate contents of caffeine in food and beverages. World J Chem Educ 5:148–152. https://doi.org/10.12691/wjce-5-5-1

Article  CAS  Google Scholar 

Merck Millipore HPTLC application number 412: quantification of caffeine in different coffee samples. https://www.merckmillipore.com/deepweb/assets/sigmaaldrich/product/documents/188/802/412-hptlc-quantification-of-caffeine-in-coffee-mb20151013-final-mk.pdf. Accessed 7 July 2025

Aranda M, Morlock G (2006) Simultaneous determination of riboflavin, pyridoxine, nicotinamide, caffeine and taurine in energy drinks by planar chromatography-multiple detection with confirmation by electrospray ionization mass spectrometry. J Chromatogr A 1131:253–260. https://doi.org/10.1016/j.chroma.2006.07.018

Article  PubMed  CAS  Google Scholar 

Abourashed EA, Mossa JS (2004) HPTLC determination of caffeine in stimulant herbal products and power drinks. J Pharm Biomed Anal 36:617–620. https://doi.org/10.1016/j.jpba.2004.06.029

Article  PubMed  CAS  Google Scholar 

Raj R, Chandrashekar KS, Biswas R, Pai A, Pai V (2021) Rapid high-performance thin layer chromatographic quantitative estimation of caffeine in various foods and beverages. Rasayan J Chem 14:221–226. https://doi.org/10.31788/rjc.2021.1415991

Article  CAS  Google Scholar 

Menguy L, Prim D, Carlin-Sinclair A, Marc I (2009) The determination of methylxanthines in chocolate and cocoa by different separation techniques: HPLC, instrumental TLC, and MECC. J Chem Educ 86:1307. https://doi.org/10.1021/ed086p1307

Article  Google Scholar 

Vovk I, Glavnik V (2015) Analysis of dietary supplements. In: Poole C (ed) Instrumental thin-layer chromatography. Elsevier, Amsterdam, pp 589–635

Google Scholar 

Vovk I, Glavnik V (2023) Applications of thin-layer chromatography to the quality control of dietary supplements. In: Poole C (ed) Instrumental thin-layer chromatography. Elsevier, Amsterdam, pp 615–663

Google Scholar 

Alam P, Shakeel F, Ali A, Alqarni MH, Foudah AI, Aljarba TM, Alkholifi FK, Alshehri S, Ghoneim MM, Ali A (2022) Simultaneous determination of caffeine and paracetamol in commercial formulations using greener normal-phase and reversed-phase HPTLC methods: a contrast of validation parameters. Molecules 27:405. https://doi.org/10.3390/molecules27020405

Article  PubMed  PubMed Central  CAS  Google Scholar 

Ragab MT, Ramadan NK, El-Ragehy NA, El-Zeany BA (2023) Thin layer chromatography-spectrodensitometric determination of a three-component mixture of propyphenazone, caffeine, ergotamine tartrate, and two of their impurities with application to tablets, spiked human plasma, and green profile assessment. JPC J Planar Chromatogr Mod TLC. 36:295–305. https://doi.org/10.1007/s00764-023-00248-x

Article  CAS  Google Scholar 

Arage A, Layloff T, Hymete A, Ashenef A (2023) High performance thin layer chromatography (HPTLC) method development and validation for the simultaneous determination of paracetamol, caffeine, chlorpheniramine and phenylepherine in tablet formulation. Acta Chromatogr 35:170–178. https://doi.org/10.1556/1326.2022.01028

Article  CAS  Google Scholar 

Lebot V, Melteras M, Pilecki A, Labouisse J-P (2020) Chemometric evaluation of cocoa (Theobroma cacao L.) and coffee (Coffea spp.) germplasm using HPTLC. Genet Resour Crop Evol 67:895–911. https://doi.org/10.1007/s10722-020-00888-6

Article  CAS  Google Scholar 

Reich E, Schibli A, Widmer V, Jorns R, Wolfram E, DeBatt A (2006) HPTLC methods for identification of green tea and green tea extract. J Liq Chromatogr Relat Technol 29:2141–2151. https://doi.org/10.1080/15512160600760293

Article  CAS  Google Scholar 

National Institute of Standards and Technology (2007) Certificate of analysis: standard reference material 2384 – baking chocolate

EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA) (2015) Scientific opinion on the safety of caffeine. Efsa J 13(5):4102

Google Scholar 

Comments (0)

No login
gif