A rapid, ecofriendly, high-performance thin-layer chromatography method for the quantification of three bioactive compounds in L.

Behera MC, Mohanty TL, Paramanik BK (2017) Silvics, phytochemistry and ethnopharmacy of endangered poison nut tree (Strychnos nux-vomica L.): A review. J Pharmacogn Phytochem 6(5):1207–1216

CAS  Google Scholar 

Mitra SW, Shukla VJ, Acharya RA (2012) Impact of Shodhan (purifactory procedures) on Kupeelu (Strychnus nux-vomica Linn) seeds: a pharmaceuticoanalytical study. J Res Edu Ind Med 27(2):65–71

Google Scholar 

Kumar MA, Jayasingh S, Ojha N et al (2012) A comprehensive review on effects of sodhan karma (detoxification procedure) and therapeutic potential of visha-tinduka (Strychnos nux-vomica). IJRAP 3(2):211–213

Google Scholar 

Eldahshan OA, Abdel-Daim MM (2015) Phytochemical study, cytotoxic, analgesic, antipyretic and anti-inflammatory activities of Strychnos nux-vomica. Cytotechnology 67:831–844. https://doi.org/10.1007/s10616-014-9723-2

Article  CAS  PubMed  Google Scholar 

Maji Amal K, Pratim B (2017) Strychnos nux-vomica: a poisonous plant with various aspects of therapeutic significance. J Basic Clin Pharm 8:S087-S103

CAS  Google Scholar 

Chalakh S, Wanjari A, Urkude M (2024) Phytochemical analysis of strychnine content of processed nux-vomica seeds in different media. Afr J Bio Sc 6(Si2):3068–3075. https://doi.org/10.33472/AFJBS.6.Si2.2024.3069-3075

Article  CAS  Google Scholar 

Bhati R, Singh A, Saharan VA et al (2012) Strychnos nux-vomica seeds: Pharmacognostical standardization, extraction, and antidiabetic activity. J Ayurveda Integr Med 3(2):80. https://doi.org/10.4103/0975-9476.96523

Article  PubMed  PubMed Central  Google Scholar 

Chitra V, Varma PV, Raju MVRK et al (2010) Study of antidiabetic and free radical scavenging activity of the seed extract of Strychnos nuxvomica. Int J Pharm Pharm Sci 2(SUPPL 1):106–110

Google Scholar 

Joy ALM, Appavoo MR (2015) Antibacterial and antifungal activity of Strychnos nux vomica seed extract. JOCPR 7(4):1495–1499

Google Scholar 

Rao PS, Ramanadham M, Prasad MN (2009) Anti-proliferative and cytotoxic effects of Strychnos nux-vomica root extract on human multiple myeloma cell line–RPMI 8226. Food Chem Toxicol 47(2):283–288. https://doi.org/10.1016/j.fct.2008.10.027

Article  CAS  PubMed  Google Scholar 

Mian SS, Alam MI, Khan NA et al (2023) Standardization of different extracts of detoxified nux-vomica seeds with its comparative study by TLC and HPTLC. J Herb Med 1(42):100792. https://doi.org/10.1016/j.hermed.2023.100792

Article  Google Scholar 

Ramachandran S, Meenatchisundaram S, Allu R et al (2022) Proximate analysis, HPTLC finger print analysis and multi spectrometric analysis of Strychnos nux-vomica nuts. J Ayurveda Integr Med 19(2):233–242. https://doi.org/10.1515/jcim-2021-0528

Article  CAS  Google Scholar 

Damle MC, Bandal P, Kale S et al (2017) HPTLC estimation and antibacterial effect of methanolic extract of S. nux vomica seeds under accelerated storage condition. PSM 8(2):69–76

CAS  Google Scholar 

Sharma DB, Aphale P, Sinnarkar V et al (2021) Standardization of homoeopathic mother tincture of Strychnous nux vomica with the help of high-performance thin layer chromatography and correlation of its alkaloid markers with its toxicological action. RJPT 14(8):4203–4206

Google Scholar 

Guo Y, Meng Z, Gu Y et al (2022) HPLC-MS/MS analysis of primary alkaloids in rat plasma after oral administration of “Nux vomica-Glycyrrhiza glabra decoction”: a pharmacokinetic study. J Ethnopharmacol 298:115588. https://doi.org/10.1016/j.jep.2022.115588

Article  CAS  PubMed  Google Scholar 

Patel B, Mashru R (2017) Development and validation of RP-HPLC Method for strychnine in nux vomica containing formulations. WJPR 6(4):1650–1658

CAS  Google Scholar 

Patil SP, Laddha KS (2017) Simple method of isolation of loganin from Nux vomica fruits and its quantification by HPLC. IJP 4(1):39–42

CAS  Google Scholar 

Van Eenoo P, Deventer K, Roels K et al (2006) Quantitative LC–MS determination of strychnine in urine after ingestion of a Strychnos nux-vomica preparation and its consequences in doping control. Forensic Sci Int 164(2–3):159–163. https://doi.org/10.1016/j.forsciint.2005.12.029

Article  CAS  PubMed  Google Scholar 

Maddisetty PN, Doss VA (2017) HPLC and GC-MS: identification and quantification of strychnine and brucine in Strychnos nux-vomica leaves. Pak J Pharm Sci 30:2369

PubMed  Google Scholar 

International Council for Harmonisation (2022) Guideline IHT. Validation of analytical procedures Q2 (R2). Geneva.

Kowtharapu LP, Katari NK, Muchakayala SK et al (2023) Green metric tools for analytical methods assessment critical review, case studies and crucify. TrAC, Trends Anal Chem 166:117196. https://doi.org/10.1016/j.trac.2023.117196

Article  CAS  Google Scholar 

Manousi N, Wojnowski W, Płotka-Wasylka J et al (2023) Blue applicability grade index (BAGI) and software: a new tool for the evaluation of method practicality. Green Chem 25(19):7598–7604. https://doi.org/10.1039/D3GC02347H

Article  CAS  Google Scholar 

Sajid M, Płotka-Wasylka J (2022) Green analytical chemistry metrics: a review. Talanta 238:123046. https://doi.org/10.1016/j.talanta.2021.123046

Article  CAS  PubMed  Google Scholar 

Chaudhary MK, Misra A, Tripathi D et al (2022) In-vitro anti-urolithiatic activity and simultaneous HPTLC quantification of berberine and palmatine in standardized extract of Thalictrum foliolosum DC. S Afr J Bot 151:445–453. https://doi.org/10.1016/j.sajb.2022.10.026

Article  CAS  Google Scholar 

Chaudhary MK, Misra A, Tiwari A et al (2025) Integrating a greener RP-HPLC method and chemical pattern recognition for the identification of Acorus calamus L. and its common adulterants in Indian markets. Anal Methods 17:1477–1486. https://doi.org/10.1039/D4AY02102A

Article  CAS  PubMed  Google Scholar 

Kamal A, Kamal YT, Ahmad S, Ahmad FJ, Saleem K (2012) Simultaneous HPTLC determination of strychnine and brucine in Strychnos nux-vomica seed. JBPS 4(2):134–139. https://doi.org/10.4103/0975-7406.94814

Article  CAS  Google Scholar 

Mitra S, Kumar V, Ashok B, Acharya RN et al (2011) A comparative anti-inflammatory activity of raw and processed Kupeelu (Strychnos nux-vomica Linn.) seeds on albino rats. Anc Sci Life 31(2):73–75

PubMed  PubMed Central  Google Scholar 

Chaudhary MK, Misra A, Srivastava S (2024) Optimization of six metabolite contents using response surface methodology in Pueraria tuberosa (Willd.) DC. tuber: RP-HPLC-PDA method validation and greenness assessment. Sustain Chem Pharm 42:101828. https://doi.org/10.1016/j.scp.2024.101828

Article  CAS  Google Scholar 

Nagieb HM, Abdelwahab NS, Abdelrahman MM et al (2023) Greenness assessment of UPLC/MS/MS method for determination of two antihypertensive agents and their harmful impurities with ADME/TOX profile study. Sci Rep 13(1):19318. https://doi.org/10.1038/s41598-023-46636-5

Article  CAS  PubMed  PubMed Central  Google Scholar 

Comments (0)

No login
gif