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Analysis of Mesoscope Imaging Data
Analysis of Mesoscope Imaging Data
Mesoscope imaging enables the recording of neural activity projections in the dorsal cortex of behaving subjects through p...
Direct or DNA Extraction-Free Amplification and Quantification of Foodborne Pathogens
Direct or DNA Extraction-Free Amplification and Quantification of Foodborne Pathogens
The use of direct nucleic acid amplification of pathogens from food matrices has the potential to reduce time to results o...
Supervised Chromatin Loop Detection Using Peakachu Version 2
Supervised Chromatin Loop Detection Using Peakachu Version 2
Peakachu is a supervised-learning-based approach that identifies chromatin loops from chromatin contact data. Here, we pre...
Phosphopeptide Enrichment and LC-MS/MS Analysis to Study the Phosphoproteome of Recombinant Chinese Hamster Ovary Cells
Phosphopeptide Enrichment and LC-MS/MS Analysis to Study the Phosphoproteome of Recombinant Chinese Hamster Ovary Cells
The reversible phosphorylation of proteins on serine, threonine, and tyrosine residues is one of the most important post-t...
Evaluation of Gene Expression Profiling by QuantiGene™ 2.0 RNA Assay
Evaluation of Gene Expression Profiling by QuantiGene™ 2.0 RNA Assay
QuantiGene™ 2.0 technique could be used to investigate the gene expression signature of the immune system senescence...
Luciferase Reporter Assay for Determining the Signaling Activity of Interferons
Luciferase Reporter Assay for Determining the Signaling Activity of Interferons
The classic dual luciferase reporter assay has been widely used to rapidly and accurately determine the transcriptional ac...
Mesoscale Imaging of Neural Dynamics in Epilepsy
Mesoscale Imaging of Neural Dynamics in Epilepsy
Epilepsy is a disease characterized by recurrent seizures. When focal in onset, these events start in a defined location a...
CE-MS-Based Clinical Metabolomics of Human Plasma
CE-MS-Based Clinical Metabolomics of Human Plasma
Capillary electrophoresis coupled to mass spectrometry (CE-MS) has emerged as a powerful analytical technique with signifi...
LC-MS/MS Quantification of Endocannabinoids in Tissues
LC-MS/MS Quantification of Endocannabinoids in Tissues
Endocannabinoids (ECBs) are lipid-derived endogenous molecules with important physiological roles such as regulation of en...
Antioxidant Features of Humic Products by ABTS Assay
Antioxidant Features of Humic Products by ABTS Assay
Oxidative stress plays a pivotal role in driving immunosenescence by disrupting cellular homeostasis and impairing immune ...
Polyphenol Treatment of Peripheral Blood Mononuclear Cells from Individuals of Different Ages
Polyphenol Treatment of Peripheral Blood Mononuclear Cells from Individuals of Different Ages
Human peripheral blood mononuclear cells (PBMCs) have been largely utilized to assess the cytotoxic, immunomodulatory, and...
Analysis and Visualization of Multiple Hi-C and Micro-C Data with CustardPy
Analysis and Visualization of Multiple Hi-C and Micro-C Data with CustardPy
Three-dimensional (3D) genome structure plays crucial roles in biological processes and disease pathogenesis. Hi-C and Mic...
Differential Mobility Spectrometry-Based Cardiolipin Analysis
Differential Mobility Spectrometry-Based Cardiolipin Analysis
Cardiolipins (CL) are special lipids in many respects. First of all, CL are composed of four fatty acids linked by two pho...
Serum-Free and Protein-Free Media for the Cultivation of Recombinant Chinese Hamster Ovary (CHO) Cell Lines
Serum-Free and Protein-Free Media for the Cultivation of Recombinant Chinese Hamster Ovary (CHO) Cell Lines
We describe the preparation of two cell culture media formulations for the culture in suspension of Chinese hamster ovary ...
The ‘Omics Revolution in CHO Biology: Roadmap to Improved CHO Productivity
The ‘Omics Revolution in CHO Biology: Roadmap to Improved CHO Productivity
Chinese hamster ovary (CHO) cell physiology understanding has advanced very rapidly in the past few years with incredible ...
Methods for Genome-Wide Chromatin Interaction Analysis
Methods for Genome-Wide Chromatin Interaction Analysis
Recent analyses revealed the essential function of chromatin structure in maintaining and regulating genomic information. ...
Transcriptomics in the Study of Antiviral Innate Immunity
Transcriptomics in the Study of Antiviral Innate Immunity
Transcriptomics is an extremely important area of molecular biology and is a powerful tool for studying all RNA molecules ...
A Guideline Strategy for Identifying Genes/Proteins Regulating Antiviral Innate Immunity
A Guideline Strategy for Identifying Genes/Proteins Regulating Antiviral Innate Immunity
Antiviral innate immunity is a complicated system initiated by the induction of type I interferon (IFN-I) and downstream i...
Epidemiological Study in Antiviral Innate Immunity
Epidemiological Study in Antiviral Innate Immunity
This chapter summarizes the epidemiological study design of natural immune epidemiology studies based on recent COVID-19-r...
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